MP201 treatment attenuates demyelination in optic nerves during EAE. To examine whether MP201 treatment prevents demyelination, optic nerves were isolated from mice 42 days post-immunization, and were stained with LFB. A. LFB stained optic nerve longitudinal sections were examined by a blinded investigator and demyelination was quantified on a 0-3 point scale. optic nerves (N=10 nerves) from EAE mice had a significantly (**p<0.01) higher demyelination score compared to optic nerves (N=10) from control mice, and treatment with 16 mg/kg (@@p<0.01 vs EAE, N=10) or 80 mg/kg (@p<0.05 vs EAE, N=10) MP201 lead to a significant decrease in demyelination scores. B. A representative image of one optic nerve from a PBS-treated EAE mouse shows less LFB (blue) staining than an optic nerve from a control mouse, as well as optic nerves of EAE mice treated with either 16 mg/kg or 80 mg/kg MP201 (original magnification X20.